| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.245921 |
| Chromosome: | chromosome 14 |
| Location: | 2388331 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g624350 | VTE6 | Tocopherol O-methyltransferase; (1 of 1) K18534 - MPBQ/MSBQ methyltransferase (K18534) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGCGGCCAACACGCAGGACCGCGTGCC |
| Internal bar code: | CCCTCGTCGAGGGTCGCAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 601 |
| LEAP-Seq percent confirming: | 98.9578 |
| LEAP-Seq n confirming: | 1899 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACTCCACAAACGACAGGG |
| Suggested primer 2: | GTCCATTACGCGGACAAGTT |