Insertion junction: LMJ.RY0402.245943_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.245943
Chromosome:chromosome 17
Location:3101243
Confidence (%):95
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGCTGGACTCGCTGTTGTAGACTTACCT
Internal bar code:GCCCTACCGCAGCCTCAGCTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:322
LEAP-Seq percent confirming:99.2806
LEAP-Seq n confirming:138
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AAACAGACCCTGCATGGAAC
Suggested primer 2:GGTCCTCATCCAGGAACTCA