| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.245949 |
| Chromosome: | chromosome 1 |
| Location: | 6214466 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044400 | (1 of 1) IPR019546 - Twin-arginine translocation pathway, signal sequence, bacterial/archaeal | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTAGGAAGCCACGACGCGACGGTGCGGGC |
| Internal bar code: | TTACCACGAGAGTCGGGAGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 50 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCATCATCAGCAGCAACAG |
| Suggested primer 2: | TCAGGTTCGTTAGCAGGCTT |