| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.245956 |
| Chromosome: | chromosome 12 |
| Location: | 4544349 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g522250 | (1 of 13) PF00350 - Dynamin family (Dynamin_N) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGAGTCGGGCGTCTCTGCACTCGTAAGG |
| Internal bar code: | TTCCGTGTGTGATAACGACTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 537 |
| LEAP-Seq percent confirming: | 95.2802 |
| LEAP-Seq n confirming: | 646 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCGTTGTGCAGATAGAAG |
| Suggested primer 2: | CATAGCTGCTACGCCAATGA |