| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.245971 |
| Chromosome: | chromosome_16 |
| Location: | 4712992 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g687000 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTTCACGCAACAGTCCTTCAAGCAAGTCT |
| Internal bar code: | AAACAGGCTCTCTTGTGTGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 564 |
| LEAP-Seq percent confirming: | 76.494 |
| LEAP-Seq n confirming: | 192 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAGCTAGTTGTGCACGTA |
| Suggested primer 2: | ACACCCAGGCCTATCCTCTT |