| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.245986 |
| Chromosome: | chromosome 8 |
| Location: | 2139142 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g369150 | (1 of 1) PTHR10566:SF50 - PROTEIN LACT-3 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCTTGGGTGGTGATGGCGGGGAGGGGAG |
| Internal bar code: | GGGCCGAGCTAGTACCCACTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 95 |
| LEAP-Seq percent confirming: | 99.4135 |
| LEAP-Seq n confirming: | 339 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGATACGAGAAAGGGGCT |
| Suggested primer 2: | CTTCGTACGCAAGTGTGCAT |