| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.246057 |
| Chromosome: | chromosome 12 |
| Location: | 2425086 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507051 | (1 of 2) K01193 - beta-fructofuranosidase (E3.2.1.26, sacA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGTTCTTCACCCTCCCGCCGTCGGCAGT |
| Internal bar code: | GCGCGGCGGATGCTCAGCGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 550 |
| LEAP-Seq percent confirming: | 21.4689 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 139 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGGGCTGGCACTTACAAC |
| Suggested primer 2: | ATGCACTCCGATCTCCATTC |