| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.246084 |
| Chromosome: | chromosome 9 |
| Location: | 4286658 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394658 | LCI28 | (1 of 1) 1.1.1.21 - Aldehyde reductase / Polyol dehydrogenase (NADP(+)); Low-CO2-induced aldose reductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTAGATGCGCGCGCAGTCGATGTGGCGG |
| Internal bar code: | TTAAGGCAATCCCGTGCACCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 883 |
| LEAP-Seq percent confirming: | 99.1639 |
| LEAP-Seq n confirming: | 7709 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGAGGAGCAAAAGCGTC |
| Suggested primer 2: | GGAACAAACCTCCCTTGACA |