Insertion junction: LMJ.RY0402.246104_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.246104
Chromosome:chromosome 9
Location:3246746
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g387763 (1 of 1) 1.1.1.94 - Glycerol-3-phosphate dehydrogenase (NAD(P)(+)) / L-glycerol-3-phosphate:NAD(P) oxidoreductase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCTATGCATGGCGCCACCGCCCAAGCAC
Internal bar code:TCGACGCTAGGAGCATTTCTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:970
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ATCGACCTTGGTCCACTCAC
Suggested primer 2:AACCCCGAAAGTCTTGTGTG