Insertion junction: LMJ.RY0402.246179_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.246179
Chromosome:chromosome 14
Location:456030
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g610900 (1 of 1) IPR026205 - Progesterone-induced-blocking factor 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGGCACCCGCTCCAACCCACATCCCACG
Internal bar code:GGGACTGGAACAATCGAGCTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:471
LEAP-Seq percent confirming:89.3333
LEAP-Seq n confirming:67
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGTCTCCCCAACACACCT
Suggested primer 2:GTAGCTGTGTGGGTCTGGGT