| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.246223 |
| Chromosome: | chromosome_1 |
| Location: | 4779232 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g033000 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGCCACTAACTCCACTCATTTCAGCAACC |
| Internal bar code: | TCGCTATTGAGTATAGTGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 296 |
| LEAP-Seq percent confirming: | 99.3045 |
| LEAP-Seq n confirming: | 2570 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATGAAGTATGACCGCCAA |
| Suggested primer 2: | TTATTGAAGAGGCACGAGGG |