| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.246305 |
| Chromosome: | chromosome_8 |
| Location: | 988371 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g362000 | FAP72 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCTTGTACCTCTTGCCCCGCTTGAAGTCT |
| Internal bar code: | TGTAGTGCGTGCTTCGCGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 600 |
| LEAP-Seq percent confirming: | 85.9069 |
| LEAP-Seq n confirming: | 4779 |
| LEAP-Seq n nonconfirming: | 784 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCGTATCCTTCAGTAAGC |
| Suggested primer 2: | TTACACAACTGCAGGCGTTC |