Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.246308 |
Chromosome: | chromosome_2 |
Location: | 5354453 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g107050 | DHC5 | Dynein heavy chain 5, inner arm dynein b (monomeric) | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CATTCCCGCTCACTCCATGCCGTCCACACC |
Internal bar code: | CTCCGGGATTTCGTAAGGGCTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 471 |
LEAP-Seq percent confirming: | 99.8745 |
LEAP-Seq n confirming: | 1591 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTTGCTCAACTCTCCCAAC |
Suggested primer 2: | GCCTGGCAACAGCTTAGAAC |