| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.246379 |
| Chromosome: | chromosome 16 |
| Location: | 2320707 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659450 | (1 of 3) 2.4.1.228 - Lactosylceramide 4-alpha-galactosyltransferase / Histo-blood group P(k) UDP-galactose | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACCGTATGCCTAGATACACGCCAGGGTC |
| Internal bar code: | TTGCTCTAGTGGCTCACTACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 674 |
| LEAP-Seq percent confirming: | 99.5245 |
| LEAP-Seq n confirming: | 3349 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCTACATGGACACCGACG |
| Suggested primer 2: | TCCACTTCCACCTCTTCCAC |