| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.246389 |
| Chromosome: | chromosome 2 |
| Location: | 1650804 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085450 | CPX1 | (1 of 2) 1.3.3.3 - Coproporphyrinogen oxidase / Coproporphyrinogenase; Coproporphyrinogen III oxidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGGCATGGCCTGTGAGTGCGATATAAA |
| Internal bar code: | TCATGCGACACAGCCGTTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 931 |
| LEAP-Seq percent confirming: | 98.5692 |
| LEAP-Seq n confirming: | 620 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCACCCTACGGGACTTTAC |
| Suggested primer 2: | AGCACATGAACGACCCCTAC |