| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.246436 |
| Chromosome: | chromosome 6 |
| Location: | 3135524 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g275300 | (1 of 3) PF05670 - Domain of unknown function (DUF814) (DUF814) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATTGGTGCTGAGCTCCCGCCCTCGCCGC |
| Internal bar code: | ATGGGTAACAAAGGAAAACTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 282 |
| LEAP-Seq percent confirming: | 97.9167 |
| LEAP-Seq n confirming: | 188 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTAGTCCAGTGCGACCAT |
| Suggested primer 2: | CTGGTCCTGGAAATGGAAGA |