| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.246458 |
| Chromosome: | chromosome 10 |
| Location: | 1512021 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428900 | RRM9,CGL72 | (1 of 1) 2.1.1.226 - 23S rRNA (cytidine(1920)-2'-O)-methyltransferase; Conserved in the Green Lineage | gene_edge/mRNA_edge/5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAACTTGTCCAGGCACGCCTTAGCCCTG |
| Internal bar code: | TTGGGAGACATGACCTGTGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 572 |
| LEAP-Seq percent confirming: | 99.1694 |
| LEAP-Seq n confirming: | 597 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCCCACAACAAAATTGA |
| Suggested primer 2: | CGCCTTCTCCATCTTGAGTC |