| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.246460 |
| Chromosome: | chromosome 2 |
| Location: | 992682 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g080300 | (1 of 5) PTHR10791 - RAG1-ACTIVATING PROTEIN 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGGATGATGGCTGTTGTGCGCGGGCGCG |
| Internal bar code: | CCCGTACTGTAGGGTCAAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 501 |
| LEAP-Seq percent confirming: | 99.1708 |
| LEAP-Seq n confirming: | 598 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTCGGATGTGTCCCTGTC |
| Suggested primer 2: | GGATCCATACTTGTGTGGGG |