| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.246478 |
| Chromosome: | chromosome 14 |
| Location: | 1821589 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g620200 | (1 of 1) IPR011527//IPR021261 - ABC transporter type 1, transmembrane domain // Protein of unknown function DUF2838 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCAGGCATCGGGGGCTCTCCACTAGTA |
| Internal bar code: | TACTTCGATTCACGACTCATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 98.9305 |
| LEAP-Seq n confirming: | 185 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCACATGCATCACACCAA |
| Suggested primer 2: | CACAGTGACCCTCTCAAGCA |