| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.246555 |
| Chromosome: | chromosome 10 |
| Location: | 291609 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419900 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGGCATTTCTTCGGCATTGAGGTCGCA |
| Internal bar code: | GTGGAGTACCTTCCATTGCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 332 |
| LEAP-Seq percent confirming: | 99.7703 |
| LEAP-Seq n confirming: | 5213 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGTTACCTAGCATGTCGT |
| Suggested primer 2: | GCGCTAATGCTTTCTTACCG |