Insertion junction: LMJ.RY0402.246563_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.246563
Chromosome:chromosome_17
Location:3094824
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre17.g721000 antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):AGTTTCTGAACACGCGCGCGCTGTCACCGC
Internal bar code:CAACGCTGTTTATTAAAAAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:228
LEAP-Seq percent confirming:76.9953
LEAP-Seq n confirming:164
LEAP-Seq n nonconfirming:49
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTTCATCTCAATCTCGC
Suggested primer 2:CAACACTCCTCACCTCCCAC