Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.246563 |
Chromosome: | chromosome_17 |
Location: | 3094824 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g721000 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGTTTCTGAACACGCGCGCGCTGTCACCGC |
Internal bar code: | CAACGCTGTTTATTAAAAAGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 228 |
LEAP-Seq percent confirming: | 76.9953 |
LEAP-Seq n confirming: | 164 |
LEAP-Seq n nonconfirming: | 49 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCTTCATCTCAATCTCGC |
Suggested primer 2: | CAACACTCCTCACCTCCCAC |