| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.246604 |
| Chromosome: | chromosome 6 |
| Location: | 6926424 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g296100 | (1 of 1) 3.6.1.29 - Bis(5'-adenosyl)-triphosphatase / Dinucleosidetriphosphatase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTGACATGCATCACTGCATGGGTTGCG |
| Internal bar code: | GTTTATCATTGCCTCTTGTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 823 |
| LEAP-Seq percent confirming: | 96.3879 |
| LEAP-Seq n confirming: | 2455 |
| LEAP-Seq n nonconfirming: | 92 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCAGAGTGCGAGAGTGAG |
| Suggested primer 2: | AACGCTGAGTTGGTGCTCTT |