| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.246644 |
| Chromosome: | chromosome 9 |
| Location: | 496239 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g404201 | PHC30,PHC24,PHC10 | Pherophorin-chlamydomonas homolog 30; (1 of 71) PF12499 - Pherophorin (DUF3707) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCGGCCTCGTGTGCCTGGAGCTGGATGG |
| Internal bar code: | TTCATCTAGCCCGGAGGAGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 916 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 108 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGAGTGACACCTATTGGC |
| Suggested primer 2: | TAGCTGGTACGTGCATCTGG |