Insertion junction: LMJ.RY0402.246690_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.246690
Chromosome:chromosome 4
Location:390931
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g217916 (1 of 1) IPR001395//IPR005399//IPR020471//IPR023210 - Aldo/keto reductase // Potassium channel, voltage-dependent, beta subunit, KCNAB-related // Aldo/keto reductase subgroup // NADP-dependent oxidoreductase domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCAGGTCCGCAAATCTCACGGCATGTGTG
Internal bar code:ATCGTACTCCCGGCTCGTTTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1065
LEAP-Seq percent confirming:99.6226
LEAP-Seq n confirming:264
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTCCGATTTGCAGACTTC
Suggested primer 2:CATCCTCTCTACCCCCTTCC