| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.246751 |
| Chromosome: | chromosome 12 |
| Location: | 8409358 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548650 | BBS4 | (1 of 1) K16531 - Bardet-Biedl syndrome 4 protein (BBS4); Bardet Biedl syndrome-4 associated protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGTCTCCGAACTGGTGTGGCGGGTGACA |
| Internal bar code: | TGTTCGTGATCGGAGCTGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 755 |
| LEAP-Seq percent confirming: | 33.6199 |
| LEAP-Seq n confirming: | 391 |
| LEAP-Seq n nonconfirming: | 772 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTTCACGACTGAGGAATC |
| Suggested primer 2: | GGTCTGTGGAGTGAGGTGGT |