| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.246793 |
| Chromosome: | chromosome 3 |
| Location: | 588959 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145547 | CBR1 | (1 of 3) K00326 - cytochrome-b5 reductase (E1.6.2.2); NADH-cytochrome b5 reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGAAAGCGTAGCGGCGTGCGTTACGTG |
| Internal bar code: | GGGCGGTAGACAAGTTAGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 709 |
| LEAP-Seq percent confirming: | 99.4526 |
| LEAP-Seq n confirming: | 545 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGAGGGTGCAAGAGTGAG |
| Suggested primer 2: | TTGGCTGTGTATTAGCTGCG |