Insertion junction: LMJ.RY0402.246810_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.246810
Chromosome:chromosome 16
Location:7179257
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g681354 (1 of 41) PF13920 - Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTGTCGGAAGTACAGCGCACCAAACACA
Internal bar code:AGAGTGAGTCTAGGAGCGCCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:136
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCCGAAAGTCTGTAAGCAGG
Suggested primer 2:ATTGTTTTGAAGCAGGGGTG