Insertion junction: LMJ.RY0402.246825_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.246825
Chromosome:chromosome 2
Location:6542073
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g116850 HLM6,SETb (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase; Histone-lysine N-methyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCATTGCCACGCCCGCCCCCACGCCAAC
Internal bar code:ACGCACGAACAGTAATGGGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:422
LEAP-Seq percent confirming:98.3871
LEAP-Seq n confirming:122
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCCTCCCACTGACTTCTT
Suggested primer 2:GAGGACATGAGGGACGGATA