| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.246854 |
| Chromosome: | chromosome 6 |
| Location: | 7424593 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g299800 | LCL1 | Long-chain-fatty-acid-CoA ligase; (1 of 1) 6.2.1.20 - Long-chain-fatty-acid--[acyl-carrier-protein] ligase / Acyl-[acyl-carrier-protein] synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAGGATGACAACTGCGTGCTTGCTGACT |
| Internal bar code: | CATCGCTGGAGTGCCAGCTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 491 |
| LEAP-Seq percent confirming: | 99.2283 |
| LEAP-Seq n confirming: | 2443 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCAGTAAGTTTGGCTGAG |
| Suggested primer 2: | CACCCCGGTGATTTTATGAC |