Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.246870 |
Chromosome: | chromosome_12 |
Location: | 115888 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g484250 | DHC1,PF9,ROC34,IDA1 | Flagellar inner arm dynein 1 heavy chain alpha (I1/f alpha) | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTTGTTGTGGTGACCGCGGCAAGTTTCAAA |
Internal bar code: | GAGCCATTAGCGTCCGCTAGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 577 |
LEAP-Seq percent confirming: | 98.7952 |
LEAP-Seq n confirming: | 82 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTGTTGCGCTCAAAGATGTC |
Suggested primer 2: | GAGCTGCTCAAGGTGAAAGG |