Insertion junction: LMJ.RY0402.246913_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.246913
Chromosome:chromosome 1
Location:315051
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g001800 MLCK1,FAP403 (1 of 4) PTHR24361//PTHR24361:SF411 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SUBFAMILY NOT NAMED; Flagellar Associated Protein 403 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAAGAGGGGAAAGGGTGGCTGGTATAGGT
Internal bar code:TACGCGAGGCAAAAAAGCTCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:277
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:154
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGTGGGGTTGAGTTGAG
Suggested primer 2:TCCCCTTCCACTTGATCTTG