Insertion junction: LMJ.RY0402.246962_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.246962
Chromosome:chromosome 6
Location:3635474
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278128 (1 of 1) IPR000953//IPR005818//IPR016197 - Chromo/chromo shadow domain // Linker histone H1/H5, domain H15 // Chromo domain-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCACCAGCTGCTTCTCCGGCAGCCACGAG
Internal bar code:GTCTCGGCGCGATGGGTCAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:494
LEAP-Seq percent confirming:96.6667
LEAP-Seq n confirming:87
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGACAACTTAGATGCGGCAG
Suggested primer 2:AAGGGGAAGGACAAGCCTAA