Insertion junction: LMJ.RY0402.247075_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.247075
Chromosome:chromosome 12
Location:2149343
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g508750 LHCA2 Light-harvesting protein of photosystem I; (1 of 24) IPR001344//IPR022796//IPR023329 - Chlorophyll A-B binding protein, plant // Chlorophyll A-B binding protein // Chlorophyll a/b binding protein domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTACAAGGGGCACCCCTGGTCCTGACGAT
Internal bar code:GTAAGTTGCCGGTAGAGCGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:270
LEAP-Seq percent confirming:98.9583
LEAP-Seq n confirming:190
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGATCAGCTTCCAGCAAC
Suggested primer 2:GAGCCCTGGAACAAGGTGTA