| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.247109 |
| Chromosome: | chromosome 11 |
| Location: | 1197263 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467692 | (1 of 5) PF07466 - Protein of unknown function (DUF1517) (DUF1517) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCCCCCCTTCCACCACCGCCTCACCAGC |
| Internal bar code: | ATCGGATCTCTAGTGATGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 772 |
| LEAP-Seq percent confirming: | 99.5441 |
| LEAP-Seq n confirming: | 655 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCCACGAGAACAAGTCCG |
| Suggested primer 2: | CCGACCTGATGACATACGTG |