| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.247116 |
| Chromosome: | chromosome 12 |
| Location: | 3111446 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499950 | CPL18 | (1 of 2) PF07819 - PGAP1-like protein (PGAP1); possible serine esterase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTGATTGCGTTTCTCCGCACTCCGACA |
| Internal bar code: | GGTGGGGGCGAAGACATTACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 590 |
| LEAP-Seq percent confirming: | 99.3614 |
| LEAP-Seq n confirming: | 1556 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATACCGTGCCTAGTTCCGA |
| Suggested primer 2: | GGTGGTGTGTGAAGACGTTG |