| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.247128 |
| Chromosome: | chromosome 16 |
| Location: | 2633968 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661900 | HEL61 | (1 of 3) PTHR24031//PTHR24031:SF232 - RNA HELICASE // ATP-DEPENDENT RNA HELICASE TDRD12-RELATED; DEAD/DEAH box helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCACCAGCCTCGTGCTTCATTCACGAGT |
| Internal bar code: | ATGTTTCCTTCGTTGACGAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 905 |
| LEAP-Seq percent confirming: | 99.7701 |
| LEAP-Seq n confirming: | 868 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTAGTATTCGCGGAGCTTG |
| Suggested primer 2: | GCGAGAGGGAGTGTAAGTGC |