| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.247137 |
| Chromosome: | chromosome 3 |
| Location: | 4002811 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171950 | PPC2,PEPC2 | (1 of 2) K01595 - phosphoenolpyruvate carboxylase (ppc); Phosphoenolpyruvate carboxylase 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGATGCTGGAAGCCACCCCTCCCCGACC |
| Internal bar code: | GTCGACGCGCAACGCGGGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 577 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCTGTCAGGAAGTGAGG |
| Suggested primer 2: | ATCGGAACAAGACCCAAGTG |