| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.247137 |
| Chromosome: | chromosome 16 |
| Location: | 431368 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g693202 | SIR1 | Ferredoxin-sulfite reductase; (1 of 2) 1.8.7.1 - Assimilatory sulfite reductase (ferredoxin) / Sulfite reductase (ferredoxin) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCTGTTAGCAGCAATCAAAGGACTATAT |
| Internal bar code: | GGACGTCCGTATTCCCGGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 200 |
| LEAP-Seq percent confirming: | 97.0992 |
| LEAP-Seq n confirming: | 636 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACCTCCTTGTAGCTGCTC |
| Suggested primer 2: | AATTGCTTTACGTTACGCGG |