| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.247158 |
| Chromosome: | chromosome 2 |
| Location: | 8600720 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTTCGAGCTACAGCATGGTGCGCGGTCT |
| Internal bar code: | CTTCGCGATGATCTAATCGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 771 |
| LEAP-Seq percent confirming: | 81.1404 |
| LEAP-Seq n confirming: | 185 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTTCGGAACTGGTTGGT |
| Suggested primer 2: | GACCTGCTTGCTTTCCTGTC |