Insertion junction: LMJ.RY0402.247162_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.247162
Chromosome:chromosome_7
Location:5176996
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g348450 antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):CCCAAAATCTTCGTATTTTGCTTGCGCGTG
Internal bar code:AGAAGATGACCTTGATAGGTGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:366
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:201
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGCTAGGTGGCATGAAGC
Suggested primer 2:GGCGGAGTGTCACGTTATTT