Insertion junction: LMJ.RY0402.247166_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.247166
Chromosome:chromosome 13
Location:2576352
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g581000 GT90F26,GT90-26 GT90 family protein 26; (1 of 11) 2.4.2.26 - Protein xylosyltransferase / Uridine diphosphoxylose-protein xylosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTTGTTCTCTTAGTACTGGTATCATTTCG
Internal bar code:TGCGGATCTGGCGTGTACATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:525
LEAP-Seq percent confirming:98.1221
LEAP-Seq n confirming:627
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AGAAGTGCCGTCACGAGAGT
Suggested primer 2:TCTCGCAGCGGGTAGTAGAT