| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.247174 |
| Chromosome: | chromosome 7 |
| Location: | 1078289 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319750 | HEL35 | (1 of 1) K14807 - ATP-dependent RNA helicase DDX51/DBP6 [EC:3.6.4.13] (DDX51, DBP6); DEAD box ATP-dependent RNA helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGAAAGGCCAGCGTGTCACGGCACTGCT |
| Internal bar code: | TTCTGATCATTTCGCTGAAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 541 |
| LEAP-Seq percent confirming: | 99.5658 |
| LEAP-Seq n confirming: | 688 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGTTGAGTCTCCTGCTC |
| Suggested primer 2: | GAGTGAGTACCGGCTGCTGT |