| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.247264 |
| Chromosome: | chromosome 17 |
| Location: | 444200 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g699000 | PAT1 | Phosphate acetyltransferase; (1 of 2) 2.3.1.8 - Phosphate acetyltransferase / Phosphotransacetylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGATGCTCCACAACGCCTCGGTCCCCTCC |
| Internal bar code: | CATATGGACCATTCCGCGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 101 |
| LEAP-Seq percent confirming: | 77.51 |
| LEAP-Seq n confirming: | 193 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAGCATGTACCGAGTGT |
| Suggested primer 2: | GCCAGTAGCGACCAAGAGTC |