Insertion junction: LMJ.RY0402.247265_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.247265
Chromosome:chromosome 16
Location:7587212
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g689423 (1 of 14) PF00892 - EamA-like transporter family (EamA); Putative UDP-galactose transporter CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGGTTGCTAGTGTTTGGCACAACAACAT
Internal bar code:CTTGATGACGGGCGCGGAAATA

Confirmation - LEAP-Seq

LEAP-Seq distance:360
LEAP-Seq percent confirming:98.6487
LEAP-Seq n confirming:292
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGTAGGCCATTGTCCAGC
Suggested primer 2:ACACACCACCCTGCCTTTAG