| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.247265 |
| Chromosome: | chromosome_16 |
| Location: | 7587212 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g689423 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGGGTTGCTAGTGTTTGGCACAACAACAT |
| Internal bar code: | CTTGATGACGGGCGCGGAAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 360 |
| LEAP-Seq percent confirming: | 98.6487 |
| LEAP-Seq n confirming: | 292 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTAGGCCATTGTCCAGC |
| Suggested primer 2: | ACACACCACCCTGCCTTTAG |