Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.247273 |
Chromosome: | chromosome 13 |
Location: | 4533044 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g602700 | SPA1 | Part of COP1/SPA complex E3 ubiquitin ligase complex; (1 of 1) K16240 - protein suppressor of PHYA-105 1 (SPA1) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAATAAACTGGTGCGGGTACGGCACGGGG |
Internal bar code: | GAGAGCGACAGTGGCACAAAGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 576 |
LEAP-Seq percent confirming: | 95.0769 |
LEAP-Seq n confirming: | 309 |
LEAP-Seq n nonconfirming: | 16 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGGTGGCTAGCTACGACTTC |
Suggested primer 2: | ATAAGTGTGTGTCCGAGCCC |