| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.247275 |
| Chromosome: | chromosome 2 |
| Location: | 2013498 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g088500 | CPLD29 | (1 of 1) 5.3.3.1 - Steroid Delta-isomerase / Steroid isomerase | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTCTTCGCCTTTGTGGTGGACTCGTGT |
| Internal bar code: | GACGTCGAATCCGTCTTCACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 442 |
| LEAP-Seq percent confirming: | 98.2301 |
| LEAP-Seq n confirming: | 111 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCGCTCACTTTGAACCTC |
| Suggested primer 2: | CAGCCCAAACTAAACCCAAA |