| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.247419 |
| Chromosome: | chromosome_6 |
| Location: | 5261812 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g282450 | PHX11 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGTGGAGGAGAGGGCGGGTGCGTGTAGCT |
| Internal bar code: | TTTACAGCTCGGGGCGGCTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 386 |
| LEAP-Seq percent confirming: | 98.7302 |
| LEAP-Seq n confirming: | 2177 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACGAACTGCCCCAATAGT |
| Suggested primer 2: | CATGGGAACCGTTTTGCTAT |