| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.247419 |
| Chromosome: | chromosome_6 |
| Location: | 5262573 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g282450 | PHX11 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCGCGCCGGCAGCAAGTGGATCTGCACAC |
| Internal bar code: | GTGGCGGCGCAAGTCCTGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 200 |
| LEAP-Seq percent confirming: | 99.7622 |
| LEAP-Seq n confirming: | 839 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACATTACAGCGAGACAT |
| Suggested primer 2: | CTCGGAGCACTACGACAACA |