| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.247422 |
| Chromosome: | chromosome 9 |
| Location: | 4087905 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393506 | HCP3 | (1 of 4) 1.7.99.1 - Hydroxylamine reductase / Hydroxylamine (acceptor) reductase; Hybrid-cluster protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGTGTTGCGCGCAAGCCTTTTGCCCTCT |
| Internal bar code: | ATGTGCACTCGCGGATGAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 778 |
| LEAP-Seq percent confirming: | 99.2053 |
| LEAP-Seq n confirming: | 749 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGGATTCGGAAAAGTTA |
| Suggested primer 2: | GGTCAGGTTGCGGTACTCAT |