| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.247478 |
| Chromosome: | chromosome 5 |
| Location: | 1853147 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241851 | (1 of 39) PTHR13078:SF53 - PROTEIN MAOC-1 | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCAATGGTGGCGTTGCATACGAAGTTGT |
| Internal bar code: | GGACGGACGAATCAGTCGAGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGTAATGACGCCATCGAG |
| Suggested primer 2: | GTTAAACTGCGTCTCGCCTC |